Science Inventory

Screening the Vast PFAS Landscape: In Vitro Toxicokinetic Testing and UPLC-MS/MS Analysis


Smeltz, M. Screening the Vast PFAS Landscape: In Vitro Toxicokinetic Testing and UPLC-MS/MS Analysis. Waters Corp Southeast Mass Spectrometry Users Virtual Meeting, Virtual, Virtual, July 21, 2019 - July 21, 2020.


This talk will be presented at the Waters Southeast Mass Spectrometry Users Meeting, which is being held virtually on July 21, 2020 . This presentation focuses on the bioanalytical analysis of in vitro toxicokinetic assays with PFAS compounds.


Per- and polyfluoroalkyl substances (PFAS) have become chemicals of concern for human health as more is learned about their widespread presence and persistence in the environment. Given the inclusion of 1,220 PFAS on the Toxic Substances Control Act inventory, the availability of in vivo toxicological data and exposure information on only a subset is inadequate to provide an understanding of the potential exposures, toxicokinetics, and toxicities across this structurally diverse domain. To address this deficiency, experimental in vitro measures of human plasma protein binding and hepatic clearance were generated across a panel of PFAS, containing carboxylic acid, sulfonate, and ether functionalities, for use in IVIVE models to predict systemic concentrations. Future work, using this data, will examine toxicokinetic differences based on functional group and may help inform risk-based chemical safety assessment.

Record Details:

Product Published Date: 07/21/2020
Record Last Revised: 09/09/2020
OMB Category: Other
Record ID: 349686