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Development of an In Vitro Human Thyroid Microtissue Model
Citation:
Deisenroth, C. Development of an In Vitro Human Thyroid Microtissue Model. LifeNet Webinar Series, RTP, NC, June 18 - 25, 2020. https://doi.org/10.23645/epacomptox.12933332
Impact/Purpose:
The presentation is for a live webinar series (June 18th and 25th) hosted by LifeNet Health that aims to communicate our recent development of a 3D human thyroid microtissue culture model to an external audience of domestic and international scientists, as well as other interested stakeholders.
Description:
The US EPA Endocrine Disruptor Screening Program (EDSP) has identified several molecular thyroid targets relevant to hormone synthesis dynamics that have been adapted to high-throughput screening (HTS) assays to rapidly evaluate the ToxCast/Tox21 chemical inventories for potential thyroid disrupting chemicals (TDCs). The uncertainty surrounding the specificity of active chemicals identified in these screens and the relevance to phenotypic effects on in vivo human TH synthesis are notable data gaps for hazard identification of TDCs. This presentation describes the development of a medium-throughput organotypic screening assay comprised of reconstructed human thyroid microtissues to quantitatively evaluate the disruptive effects of chemicals on TH production and secretion. The assay provides a novel resource for scientists interested in endocrine toxicology, drug and chemical testing, or thyroid disease modeling.
URLs/Downloads:
DOI: Development of an In Vitro Human Thyroid Microtissue Model
2020-05-20_EPA_DEISENROTH_THYROID OCM_V5.PDF (PDF, NA pp, 4386.666 KB, about PDF)