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Differential expression of coding and non-coding RNAs in propranolol-exposed fathead minnow larvae
Citation:
Toth, G., D. Bencic, R. Flick, W. Huang, AND A. Biales. Differential expression of coding and non-coding RNAs in propranolol-exposed fathead minnow larvae. 2019 SETAC North America Annual Meeting, Toronto, CANADA, November 03 - 07, 2019.
Impact/Purpose:
Poster presented at the 2019 SETAC North America Annual Meeting.
Description:
Propranolol, a beta-blocker used for high blood pressure, is one of several pharmaceuticals which have been found in freshwater aquatic resources even after municipal sewage treatment. It presents itself as a model compound for development of indicators of exposure to and effects on aquatic organisms. For decades, differential expression of messenger RNA in health and toxicological studies has been shown to be an indicator of exposure and to explain some variation in changes in apical outcomes in humans and in test organisms. Other RNA mechanisms are continuing to show evidence of greater involvement in transcription regulation than previously thought (e.g. piwi-interacting RNAs and micro-RNAs are appearing in unforeseen mRNA regulatory activities). The potential exists for new toxicological studies with these indicators to both diagnose internal exposures to specific chemical classes and to reduce unexplained variation in apical endpoints. Here, we describe a study on differential expression of mRNA, microRNA, and piwi-interacting RNA in fathead minnow (Pimephales promelas) larvae. 48 h post-hatch FHM larvae were exposed to propranolol (@ 100 ug/l – 5 mg/l) for 48 h. Lexogen Small RNA-Seq Library preparations were done, followed by Hi-Seq RNA-seq analysis. miRNA analyses were done using the miRDeep* program while piRNA analyses were conducted using the Piano and piRNN programs. Differential expression of the smRNAs was analyzed using edgeR. These results will be presented in the overall context of developing and characterizing exposure and effects biomarkers for propranolol.