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20191117 - Evaluation of the utility of ToxCast HTS and high-throughput toxicokinetic data for food chemical safety risk assessment via comparison with in vivo animal data (ACT)
Citation:
Turley, A., J. Zang, K. Friedman, R. Judson, AND S. Fitzpatrick. 20191117 - Evaluation of the utility of ToxCast HTS and high-throughput toxicokinetic data for food chemical safety risk assessment via comparison with in vivo animal data (ACT). American College of Toxicology 40th annual meeting, Phoenix, AZ, November 17 - 20, 2019. https://doi.org/10.23645/epacomptox.11310602
Impact/Purpose:
Abstract and poster for the American College of Toxicology 40th annual meeting in November 2019. New approach methodologies to traditional animal testing are currently being developed and evaluated for use in chemical safety risk assessment, including chemicals used in food. The goal of the present study is to evaluate the utility of ToxCast/Tox21 HTS data in food safety risk assessment.
Description:
New approach methodologies to traditional animal testing are currently being developed and evaluated for use in chemical safety risk assessment, including chemicals used in food. These alternatives include in vitro high-throughput screening (HTS) assays, such as the ToxCast and Tox21 assays. The ToxCast/Tox21 assays have been run thousands of compounds, including hundreds of compounds used in food, which have previously been identified and evaluated for bioactivity in these assays. However, the relationship of these types of data with traditional in vivo animal data, and the utility of these data for risk assessment remains under evaluation. The goal of the present study is to evaluate the utility of ToxCast/Tox21 HTS data in food safety risk assessment. To do this, concentrations of a subset of food-use compounds demonstrating bioactivity in ToxCast were converted to oral equivalent doses (OEDs) via in-vitro in-vivo extrapolation (IVIVE) using either in vitro or in silico-based toxicokinetic parameters for a subset of food-use compounds. These OEDs were then compared to doses demonstrated to cause effects in in vivo animal tests (using data compiled by EPA and FDA). Initial comparisons demonstrated great variability in the correlation between ToxCast and in vivo data, so steps are being taken to further refine the toxicokinetic information, chemical groups, and in vivo endpoints in an effort to identify additional information and conditions necessary to utilize HTS data for preliminary food safety assessment. This work does not reflect the official policy of the US EPA.
URLs/Downloads:
DOI: 20191117 - Evaluation of the utility of ToxCast HTS and high-throughput toxicokinetic data for food chemical safety risk assessment via comparison with in vivo animal data (ACT)
TURLEY JUDSON 2019 ACT TOXCAST FOOD ADDITIVE PODS POSTER.PDF (PDF, NA pp, 348.405 KB, about PDF)