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A disposable acetylcholine esterase sensor for As(iii) determination in groundwater matrix based on 4-acetoxyphenol hydrolysis
Citation:
Li, T., J. Berberich, E. Sahle-Demessie, AND E. Varughese. A disposable acetylcholine esterase sensor for As(iii) determination in groundwater matrix based on 4-acetoxyphenol hydrolysis. Analytical Methods. RSC Publishing, Cambridge, Uk, 11(40):5203-5213, (2019). https://doi.org/10.1039/C9AY01199D
Impact/Purpose:
Disposable amperometric sensor is a desirable tool for on-site analysis of environmental pollutants. It is cheap, quantitative, robust, and does not require special training to use.Therefore, it holds the promise to meet the need to for big data to monitor and understand the origin, migration, distribution, and transformation of environmental pollutants. Enzyme inhibition based biosensor is a special category in amperometric sensor. It combines the sensitivity of electrochemistry with specificity of enzyme inhibition. This platform has been tested for many pollutants, but still requires technical advancement for market-ready applications.
Description:
There is a lack of field compatible analytical methods for the speciation of As(III) to characterize groundwater pollution at anthropogenic sites. To address this issue, an inhibition-based acetylcholine esterase (AchE) sensor was developed to determine As(III) in groundwater. 4-Acetoxyphenol was employed to develop an amperometric assay for AchE activity. This assay was used to guide the fabrication of an AchE sensor with screen-printed carbon electrode. An As(III) determination protocol was developed based on the pseudo-irreversible inhibition mechanism. The analysis has a dynamic range of 2–500 μM (150–37 500 μg L−1) for As(III). The sensor exhibited the same dynamic range and sensitivity in a synthetic groundwater matrix. The electrode was stable for at least 150 days at 22 ± 2 °C.
URLs/Downloads:
DOI: A disposable acetylcholine esterase sensor for As(iii) determination in groundwater matrix based on 4-acetoxyphenol hydrolysis
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