Cell-Based Lipidomics for Assessing Ecological Impacts of Environmental Surface Waters
Citation:
Teng, Q., H. Zhen, Y. Yue, J. Mosley, D. Ekman, P. Bradley, D. Villeneuve, J. Cavallin, G. Ankley, AND T. Collette. Cell-Based Lipidomics for Assessing Ecological Impacts of Environmental Surface Waters. 2019 SETAC North America Annual Meeting, Toronto, November 03 - 07, 2019.
Impact/Purpose:
Presented at 2019 SETAC North America Annual Meeting
Description:
Chemical contaminants released into environmental surface waters by waste water treatment plants (WWTPs) and industrial and agricultural operations often produce adverse health outcomes in both humans and wildlife. Traditional methods using live animals for environmental monitoring and conventional risk assessments are both resource and time intensive and thus not sustainable in the long term. Cell-based assays have been widely utilized to gain information on impacted cellular pathways and relevant biomarkers of environmental exposure. In the current study, we applied a zebrafish liver (ZFL) cell-based lipidomics approach to assess the potential ecotoxicological impacts of complex contaminant mixtures in eight stream waters collected across the United States. ZFL cells were exposed to media prepared with the surface water samples for 48h. Intracellular lipophilic metabolites were detected using liquid chromatography high-resolution mass spectrometry (LC- HR MS). Untargeted lipid profiling revealed significant cellular stress resulting from exposure to several of the sites, including peroxisomal-mediated oxidative stress, endoplasmic reticulum stress, lysosomal disruption, and mitochondrial disfunction. The results demonstrate the utility of cell-based lipidomics for environmental monitoring and risk assessments of surface waters. Detailed methods and results will be reported.