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Assaying Spontaneous Network Activity and Cellular Viability using Multi-well Microelectrode Arrays
Citation:
Brown, J., B. Lynch, I. Curry-Chisolm, Tim Shafer, AND J. Strickland. Assaying Spontaneous Network Activity and Cellular Viability using Multi-well Microelectrode Arrays. METHODS IN MOLECULAR BIOLOGY. Humana Press Incorporated, Totowa, NJ, 1601:153-170, (2017). https://doi.org/10.1007/978-1-4939-6960-9_13
Impact/Purpose:
These approaches provide methods to assess compound effects on cell health and neural network function in the same well. They provide improved cell health assessment because they come from the same well as compound effects on neural network activity are being assessed. It furthers Agency goals to develop methods to screen compounds for the potential to cause developmental neurotoxicity (CSS Research). For both the Agnecy and the field, these methods save time, money and animals for high-throughput screening by eliminating “sister plates” for cell health assessments.
Description:
Microelectrode array (MEA) technology is a neurophysiological method that allows for the spontaneous measure of activity in neural cultures and determination of drug and chemical effects thereon. Recent introduction of multi-well MEA (mwMEA) formats have dramatically increased the throughput of this technology, allowing more efficient compound screening. Rapid characterization of compounds for neuroactivity or neurotoxicity hazard evaluation following acute, chronic, or developmental exposures ideally would also consider compound effects on cell health, and to do so in the same well requires a multiplexed approach. Procedures describing the multiplexed method to acute and developmental screening are described in this chapter.
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DOI: Assaying Spontaneous Network Activity and Cellular Viability using Multi-well Microelectrode Arrays