Office of Research and Development Publications

Genome-enabled insights into the ecophysiology of the comammox bacterium Ca. Nitrospira nitrosa

Citation:

Camejo, P., J. Santodomingo, K. McMahon, AND D. Noguera. Genome-enabled insights into the ecophysiology of the comammox bacterium Ca. Nitrospira nitrosa. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, 2(5):1-16, (2017). https://doi.org/10.1128/mSystems.00059-17

Impact/Purpose:

The genomes of two commamox species was generated from metagenomic data and analyzed to unveil the pathways involved in ammonia oxidation. Samples were originally obtained from a Biological Nutrient Removal (BNR) lab-scale reactor operated under alternating anaerobic and low oxygen cycles.

Description:

The recently discovered comammox bacteria have the potential to completely oxidize ammonia to nitrate. These microorganisms are part of the Nitrospira genus and are present in a variety of environments, including Biological Nutrient Removal (BNR) systems. However, the physiological traits within and between comammox- and nitrite oxidizing bacteria (NOB)-like Nitrospira species have not been analyzed in these ecosystems. In this study, we identified Nitrospira strains dominating the nitrifying community of a sequencing batch reactor (SBR) performing BNR under micro-aerobic conditions. We recovered metagenomes-derived draft genomes from two Nitrospira strains: (1) Nitrospira sp. UW-LDO-01, a comammox-like organism classified as Ca. Nitrospira nitrosa and (2) Nitrospira sp. UW-LDO-02, a nitrite oxidizing strain belonging to the Nitrospira defluvii species. A comparative genomic analysis of these strains with other Nitrospira-like genomes identified genomic differences in Ca. N. nitrosa mainly attributed to each strains’ niche adaptation. Traits associated with energy metabolism also differentiate comammox from NOB-like genomes. We also identified several transcriptionally regulated adaptive traits, including stress tolerance, biofilm formation and micro-aerobic metabolism, which might explain survival of Nitrospira under multiple environmental conditions. Overall, our analysis expanded our understanding of the genetic functional features of Ca. Nitrospira nitrosa, and identified genomic traits that further illuminates the phylogenetic diversity and metabolic plasticity of the Nitrospira genus.

URLs/Downloads:

https://doi.org/10.1128/mSystems.00059-17   Exit

Record Details:

Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Product Published Date: 10/10/2017
Record Last Revised: 03/19/2019
OMB Category: Other
Record ID: 344451

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL RISK MANAGEMENT RESEARCH LABORATORY

WATER SYSTEMS DIVISION