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Assessing Environmental Surface Water Exposure by Cell Culture-based Metabolomics
Yue, Y., J. Mosley, P. Brandley, J. Cavallin, Dan Villeneuve, G. Ankley, D. Ekman, Tim Collette, AND Q. Teng. Assessing Environmental Surface Water Exposure by Cell Culture-based Metabolomics. 2018 SETAC NA Annual Meeting, Sacramento, CA, November 04 - 08, 2018.
In this study, we applied cell culture-based metabolomics to investigate impacted biological pathways by chemical contaminants in surface water samples collected from 8 stream sites. The cell-based assay results of environmental surface waters from eight sites indicated changes in site-specific biological pathways, which in general agreed with the total number and overall concentrations of detected chemicals.
The presence of anthropogenic contaminants in environmental surface waters often results in potential health risks for humans and wildlife. As promising approaches, ’omics techniques have been developed to assess environmental risk and evaluate potential toxic impacts of chemical contaminants. Metabolomics is the ‘omics approach to understanding cell and system level biology, and has been applied in vitro for monitoring biological impacts of water contaminants and aquatic environmental stressors. Combined with high-resolution liquid chromatograph mass spectrometry (HR LC-MS), metabolomics can investigate endogenous metabolites of organisms and cells, and their significant variations under chemical exposure for discovering potential biomarkers, elucidating metabolic pathway alternations, and assessing biological impacts of chemical exposure. In this study, we applied cell culture-based metabolomics to investigate impacted biological pathways by chemical contaminants in surface water samples collected from 8 stream sites. These 8 sites were selected from 38 stream sites collected nationwide with different land occupations and chemical contaminated status, based on the number of detected chemicals in the surface water samples. Two human cell lines (liver cell, HepG2 and brain cell, LN229) and one fish cell line (zebrafish liver cell, ZFL) were exposed to these surface water samples for 48 hr. HR LC-MS was applied to investigate the impacts of chemical contaminants on endogenous polar metabolomes and biological pathways of the cells. The results provide insights into the ecological impacts of environmental contaminants on water ecology risk through a molecular-based evaluation.
Record Details:Record Type: DOCUMENT (PRESENTATION/POSTER)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL EXPOSURE RESEARCH LABORATORY
EXPOSURE METHODS & MEASUREMENT DIVISION