Science Inventory

Differential Expression of microRNAs in Fathead Minnow Larvae Exposed to Ethinyl Estradiol

Citation:

Toth, Greg, D. Lattier, D. Bencic, M. Kostich, R. Flick, AND A. Biales. Differential Expression of microRNAs in Fathead Minnow Larvae Exposed to Ethinyl Estradiol. 2018 SETAC NA Annual Meeting, Sacramento, California, November 04 - 08, 2018.

Impact/Purpose:

Unique step in development of molecular biological indicators of stressor exposure in aquatic species. Indicator is potentially integratable with other current exposure indicators (mRNA transcript expression). Data can be applied to adverse outcome pathway study.

Description:

Product is an abstract for the 2018 SETAC Meeting in Sacramento, CA in November 2018. The abstract describes the importance of studying microRNA molecules in fathead minnow (Pimephales promelas), an aquatic model organism for developing ecological exposure indicators in the laboratory setting and in the field. Fathead minnow larvae were exposed for 48 hours to 10 ng/l ethinyl estradiol, after which RNA was extracted, copied and put through high throughput sequencing (RNA-seq). microRNA molecules were identified using the program miRDeep*. Differential expression of microRNAs resulting from EE2 exposure was analyzed.

URLs/Downloads:

https://sacramento.setac.org/   Exit

Record Details:

Record Type: DOCUMENT (PRESENTATION/SLIDE)
Product Published Date: 11/08/2018
Record Last Revised: 02/15/2019
OMB Category: Other
Record ID: 344058

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL EXPOSURE RESEARCH LABORATORY

EXPOSURE METHODS & MEASUREMENT DIVISION

INTERNAL EXPOSURE INDICATORS BRANCH