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FORMATION AND PERSISTANCE OF DNA ADDUCTS IN THE LIVER OF BROWN BULLHEADS EXPOSED TO BENZO(A)PYRENE
Citation:
Sikka, H., J. Rutkowski, C. Kandaswami, S. Kumar, K. Earley, AND R. Gupta. FORMATION AND PERSISTANCE OF DNA ADDUCTS IN THE LIVER OF BROWN BULLHEADS EXPOSED TO BENZO(A)PYRENE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-90/087.
Description:
The formation and persistence of benzo[a]pyrene (BP)-DNA adducts in the liver of brown bullheads (Ictalurus nebulosus) treated with the hydrocarbon (20 mg/kg body wt, i.p.) was investigated using the 32P-postlabeling assay. he highest level of covalent binding of BP to liver DNA (188 fmol BP adducts/mg DNA) was observed 25-30 days following treatment. fter 70 days, the adduct level in liver DNA had declined to approximately 26% of the maximum adduct level. ne major BP-DNA adduct and several minomr ones were detected in the liver. he major adduct co-chromatographed with anti-BP-7,8-diol-9,10-eposide-deocyguanosine (anti-BPDE-dGuo) adduct. he data suggest that brown bullheads metabolically activate BP by the same mechanism as the mammalian systems susceptible to carcinogenic effects of the hydrocarbon.