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Multi-Method Assessment of PVP-Coated Silver Nanoparticles and Artificial Sweat Mixtures
Citation:
Peloquin, D., T. Luxton, AND E. Baumann. Multi-Method Assessment of PVP-Coated Silver Nanoparticles and Artificial Sweat Mixtures. International Conference on the Environmental Exposure of Nanoparticles and Nanomaterials 2018, Raliegh,NC, September 04 - 07, 2018.
Impact/Purpose:
Engineered nanomaterials are increasingly being incorporated into consumer products. The majority of research on exposure impacts has not taken into account how nanomaterials will be transformed based on the life cycle of the nanoparticles. Metal and metal oxide nanoparticles may undergo significant transformation throughout the product lifetime. Failure to consider how materials are transformed will lead to incorrect assumptions about what sensitive populations are being exposed to. The current research uses silver nanoparticles as a case study for understanding how interaction of the material with synthetic perspiration alters the physicochemical properties of the silver nanoparticle.
Description:
Research regarding exposure and toxicity of nanomaterials (NMs) to humans has been widely studied over the past two decades and whether engineered NMs produce adverse outcomes/responses when exposed to various microorganisms, tissues, and cells. However, the majority of research conducted on the topic has failed to consider how the immediate local environment and usage scenario impact the physical and chemical properties of NMs and how those alterations impact exposure. The current research presented utilizes silver nanoparticles (AgNPs) as a case study for how the immediate local environment alters the physical and chemical properties of NMs. The primary route for exposure to many of the consumer products containing AgNPs would occur dermally, thus nanosilver exposure in these cases must take into account interactions such as that between human sweat and AgNPs. This interaction represents an important local environment that could alter NM speciation and the specific chemical/material that individuals would be exposed to as a basic assessment of how perspiration alters the physical and chemical properties of AgNPs in a simple mixture with artificial sweat. In the current study, we utilized four different formulations of artificial sweat mixed with ionic silver and four different sizes of AgNPs. Samples were analyzed at four time intervals using dynamic light scattering (DLS), UV-Vis spectroscopy, and single particle inductively coupled plasma-mass spectrometry (spICP-MS). For the water-only stock solutions and the basic sweat mixtures, all three techniques showed a relative invariance in size over the time series, but provided different equivalent diameters from each other. With the acidic sweat mixtures, results were much more inconsistent and primarily indicated changes in either speciation or stability. Thus, each mixture was additionally centrifuged using 3 kDa filters to assess speciation changes using X-ray absorption spectroscopy.