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Filling in the Gaps: The role functional genomics can play in 21st century toxicology for environmental risk assessment
Citation:
Houck, K. Filling in the Gaps: The role functional genomics can play in 21st century toxicology for environmental risk assessment. Presented at National Academy of Science Workshop, Washington, DC, January 10 - 11, 2018. https://doi.org/10.23645/epacomptox.7029011
Impact/Purpose:
Presentation at National Academy of Science workshop in Jan 2018. Functional genomics techniques cover a comprehensive biological space and provide a functional endpoint to support involvement of genes and pathways in toxicological effects.
Description:
Chemical screening programs such as Tox21 and ToxCast have demonstrated the value of using quantitative, high-throughput assays to produce data for chemical prioritization and regulatory decisions. While these efforts have covered an impressive amount of biological space, with over 1000 endpoints analyzed in ToxCast, these approaches are limited by our incomplete knowledge of toxicity pathways and gene targets. Addressing these data gaps using high-throughput transcriptomics is an increasingly affordable option; however, it can be a challenge to identify the pathways critical to toxicity from a multitude of observed gene transcription changes. Functional genomics techniques cover a comprehensive biological space and provide a functional endpoint to support involvement of genes and pathways in toxicological effects. With the advent of CRISPR-Cas9 technology, functional genomic approaches in human cells are well positioned to increase the scope and applicability of high-throughput screening efforts supporting chemical safety assessment. This abstract does not necessarily reflect U.S. EPA policy.
URLs/Downloads:
DOI: Filling in the Gaps: The role functional genomics can play in 21st century toxicology for environmental risk assessment
NAS_2017_V1.PDF (PDF, NA pp, 2111.835 KB, about PDF)