Retrofitting an Estrogen Receptor Transactivation Assay with Metabolic Competence Using Alginate Immobilization of Metabolic Enzymes (AIME)
Citation:
DeGroot, D. AND C. Deisenroth. Retrofitting an Estrogen Receptor Transactivation Assay with Metabolic Competence Using Alginate Immobilization of Metabolic Enzymes (AIME). Presented at RIVM Netherlands National Institute for Public Health and the Environment EPA Visit, RTP, NC, April 18, 2018. https://doi.org/10.23645/epacomptox.6879689
Impact/Purpose:
Presentation at RIVM (Netherlands National Institute for Public Health and the Environment) visit to EPA on April 18, 2018
Description:
The ToxCast program has faced scrutiny as the majority of the HTS assays are deficient for xenobiotic metabolism. The challenge is to develop a method that is capable of retrofitting existing cell-free and cell-based high-throughput screening assays with metabolic competence. Risk Assessments are based upon being able to identify hazards, quantify toxicity, and measure exposure. Increasing the capabilities of alternative methods to identify hazards and assess toxicities is a critical step to incorporating these methods into risk assessment.
URLs/Downloads:
DOI: Retrofitting an Estrogen Receptor Transactivation Assay with Metabolic Competence Using Alginate Immobilization of Metabolic Enzymes (AIME)
4-18-18_RIVM_DEISENROTH_SIMMONS_METABOLISM_V2.PDF (PDF, NA pp, 1521.979 KB, about PDF)