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Interspecific and intraspecific diversity and interaction of cyanobacteria throughout a bloom
Citation:
Zhu, B., H. Cao, W. Du, G. Xu, G. Rosas, N. Xu, S. Duan, AND J. Lu. Interspecific and intraspecific diversity and interaction of cyanobacteria throughout a bloom. ASM Microbe 2018, Atlanta, GA, June 07 - 11, 2018.
Impact/Purpose:
Cyanobacterial blooms are global harmful hazards. A long-suspected question is whether dominant cyanobacterial species also has high intraspecific and/or interspecific diversity. Using high throughput 16S rRNA gene sequencing of samples from a complete bloom cycle in Harsha Lake (Ohio, USA), we found main phyla Cyanobacteria, Proteobacteria, Bacteroidetes, Actinoacteria, and Verrucomicrobia were more abundant in blooming than pre-bloom stage, while Planctomycetes showed an opposite change at five locations.
Description:
Using high throughput 16S rRNA gene sequencing of samples from a complete bloom cycle, Cyanobacteria had five main genera, Planktothrix, Aphanizomenon, Dolichospermum, Microcystis and Cylindrospermopsis, along with three minor genera Anabaena, Oscillatoria, and Leptolyngbya. Many (25% - 42%) genera were positively or negatively correlated. On an intergenic level, Planktothrix, Dolichospermum, Aphanizomenon, and Microcystis each had at least two species, giving 6 to 13 total cyanobacterial species. Most importantly, dominant species had the highest intra-specific diversity. Planktothrix rubescens, Dolichospermum flosaquae, and Aphanizomenon issatschenkoi each had at least 10 strains, while non-dominant species only had one or several strains. Most strains behaved differently throughout the bloom cycle, which were partially due to nitrogen and phosphorus concentrations. These high inter- and intra-specific diversity and intraspecific strains observed during a complete bloom cycle provides the foundation for the development of more precise bloom control strategies.
