In Vitro Toxicity Screening Technique for Volatile Substances Using Flow-Through System@@
Citation:
Winters, B., M. Angrish, A. Wallace, M. Madden, AND J. Pleil. In Vitro Toxicity Screening Technique for Volatile Substances Using Flow-Through System@@. NC SOT, RTP, NC, October 30, 2017.
Impact/Purpose:
This Novel cell culture system simplifies collection of cellular volatile substances for subsequent analysis for toxicity testing;Monitoring for perturbations of cellular volatiles, specifically metabolites of well-elucidated enzyme pathways, may be a viable method for identifying alterations in enzyme activity and be potentially used in high through put screening.
Description:
In 2007, the National Research Council envisioned the need for inexpensive, rapid, cell-based toxicity testing methods relevant to human health. in vitro screening approaches have largely addressed these problems by using robotics and automation. However, the challenge is that many of these chemicals are volatile and not amenable to liquid-based assays. We assembled an in vitro cell culture apparatus capable of screening volatile chemicals for toxicity using a metabolomics-based approach with potential for miniaturization for higher throughput.