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Targeted and Non-Targeted Analysis of Serum Pools to Provide Chemical Exposure Data for Exposure Modeling and Chemical Prioritization
Citation:
Wetmore, B., J. Wambaugh, K. Favela, A. Yau, S. Hays, R. Church, A. Rice, S. Garantziotis, AND L. Aylward. Targeted and Non-Targeted Analysis of Serum Pools to Provide Chemical Exposure Data for Exposure Modeling and Chemical Prioritization. 2018 SOT Annual Meeting and ToxExpo, TX, San Antonio, March 11 - 15, 2018.
Impact/Purpose:
This poster describes an effort to evaluate the feasibility of using pooled serum from biomonitoring studies to provide a more extensive chemical exposure characterization than currently provided in efforts such as the National Health and Nutrition Examination Survey (NHANES), where individual samples are analyzed in a targeted analysis to assess exposure a discrete set of chemicals. Targeted and non-targeted mass spectrometric analyses were conducted across 16 pools of serum that were stratified based on sex and age. Reproducibility of the data generated using pooled serum was compared to existing data on individual serum samples, and a list of chemicals of interest was generated from the non-targeted analysis. This preliminary work does indicate the feasibility of such a pooling and multi-tiered (ie, targeted and non-targeted) analysis approach. Data generated in this manner can be used to inform development of high-throughput exposure models and design of further explorations of human chemical exposure through analysis of human serum samples. Such an approach is aligned with efforts underway in the Chemical Safety for Sustainability Program to provide high-throughput exposure data that could potentially be used in risk-based prioritization.
Description:
Biomonitoring data can help inform the development and calibration of high-throughput exposure modeling for use in prioritization and risk evaluation. A pilot project was conducted to evaluate the feasibility of using pooled banked blood samples to generate initial data on population blood concentrations for compounds not to date routinely assessed in biomonitoring efforts such as the National Health and Nutrition Examination Survey (NHANES). Serum samples were obtained from the National Institute of Environmental Health Sciences (NIEHS) Clinical Research Unit. Serum pools were constructed from 25 individual 1 ml aliquots. Four pools each were constructed based on samples stratified by age (45 having a mean of 0.24 (sd 0.06) and age ≤45 having mean 0.17 (0.04). Concentrations of DEP and triclosan in serum were similar to those previously reported in other studies. Non-targeted GC analysis found an average of 355 detections per sample. Females had a significantly higher (p=0.00018) number of detections (mean 440, sd 89) than males (mean 270, sd 26). These data can be used to inform development of high-throughput exposure models and design of further explorations of human chemical exposure through analysis of human serum samples.
