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Quantification of the methane concentration using anaerobic oxidation of methane coupled to extracellular electron transfer
Gao, Y., H. Ryu, B. Rittmann, A. Hussain, AND H. Lee. Quantification of the methane concentration using anaerobic oxidation of methane coupled to extracellular electron transfer. Bioresource Technology. Elsevier Online, New York, NY, 241:979-984, (2017). https://doi.org/10.1016/j.biortech.2017.06.053
A biofilm anode acclimated with acetate, acetate+methane, and methane growth media for over three years produced a steady current density of 1.6-2.3 mA/m^2 in a microbial electrochemical cell (MxC) fed with methane as the sole electron donor. Geobacter was the dominant genus for the bacterial domain (93%) in the biofilm anode, while methanogens (Methanocorpusculum labreanum and Methanosaeta concilii) accounted for 82% of the total archaeal clones in the biofilm. A fluorescence in situ hybridization (FISH) image clearly showed a biofilm of bacteria and archaea, supporting a syntrophic interaction between them for performing anaerobic oxidation of methane (AOM) in the biofilm anode. Measured cumulative coulombs correlated linearly to the methane-gas concentration in the range of 10% to 99.97% (R^2 ≥ 0.99) when the measurement was sustained for at least 50 min. Thus, cumulative coulombs over 50 min. could be used to quantify the methane concentration in gas samples.
This work focused on relatively high methane concentration as a proof-of-concept study. The goals of this study were three-fold. The first objective was to confirm that the coupling of AOM and EET to an MxC anode can generate stable current; the second objective was to identify the microorganisms critical for performing AOM+EET in the biofilm anode; and the final objective was to assess an AOM-EET MxC as a methane sensor.This work is the first to prove that MxCs can measure the methane concentration, which is essential for monitoring methane-emission potential in an efficient and sustainable manner.
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Record Details:Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL RISK MANAGEMENT RESEARCH LABORATORY
WATER SYSTEMS DIVISION
DRINKING WATER SYSTEMS BRANCH