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Assessing the biological impact of exposure to environmental surface waters with cell-based lipidomics
Citation:
Zhen, H., J. Mosley, D. Ekman, Tim Collette, J. Cavallin, Dan Villeneuve, G. Ankley, P. Bradley, AND Q. Teng. Assessing the biological impact of exposure to environmental surface waters with cell-based lipidomics. 2017 SETAC North America Annual Meeting, Minneapolis, MN, November 12 - 16, 2017.
Impact/Purpose:
Poster presented at the SETAC North America 38th Annual Meeting
Description:
Environmental surface waters often contain a variety of chemical contaminants from different sources including wastewater treatment plants, concentrated animal feeding operations, agricultural runoff and other human-related activities. Exposure to these contaminants may pose a threat to human health and the health of wildlife (e.g. fish). We have reported the use of cell-culture based exposures in monitoring the ecotoxicological effects of chemicals in the aquatic environment and our previous focus has been mainly on changes in polar and semi-polar metabolites (i.e. the metabolome) following exposure. In the current study, we extend this approach by focusing on changes in endogenous lipophilic compounds (i.e. the lipidome) of cells in response to contaminant exposure. Two human cell lines (HepG2 and LN229) and one fish cell line (ZFL) were exposed to surface water collected from 8 streams nationwide. These sites were selected from a larger study encompassing 38 sites that cover a variety of land-use types and contamination status. After a 48 hr exposure, the lipidome of control and exposed cells was profiled with Ultra-High Performance Liquid Chromatography coupled to a Q-Exactive Orbitrap Mass Spectrometer (Thermo Scientific). We analyzed the perturbed lipid metabolism pathways and identified potential biomarkers following the exposure to environmental contaminants. In addition, we used partial least-squares regression models to explore covariances among measured chemicals in the surface waters and the lipidomics dataset, providing insights into the ecological impacts and thus determining relevance of potential environmental contaminants.
