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Summary of the development the US Environmental Protection Agency’s Medaka Extended One Generation Reproduction Test (MEOGRT) using data from 9 multigenerational medaka tests
Citation:
Flynn, K., D. Lothenbach, F. Whiteman, D. Hammermeister, L. Touart, J. Swintek, N. Tatarazako, Y. Onishi, T. Iguchi, AND R. Johnson. Summary of the development the US Environmental Protection Agency’s Medaka Extended One Generation Reproduction Test (MEOGRT) using data from 9 multigenerational medaka tests. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, 36(12):3387-3403, (2017).
Impact/Purpose:
The manuscript presents a summary of nine medaka multigenerational tests conducted both by the USEPA and Japan Ministry of the Environment. The data from these tests were used to iteratively modify test protocols that eventually became the Medaka Extended One Generation Test (MEOGRT), a Tier 2 test in the USEPA’s Endocrine Disruption Screening Program and an accepted test in the OECD Test Guideline Program. The rationale for key decisions in the development of the MEOGRT protocol are discussed along with the data supporting these decisions.
Description:
In response to various legislative mandates the United States Environmental Protection Agency (USEPA) formed its Endocrine Disruptor Screening Program (EDSP), which in turn, implemented a tiered testing strategy to determine the potential of pesticides, commercial chemicals, and environmental contaminants to disrupt the endocrine system. The first tier of tests is intended to detect the potential for endocrine disruption, specially mediated through estrogen, androgen or thyroid pathways, while the second tier is intended to further characterize the effects on these pathways and to establish a dose-response relationship for adverse effects. One of these Tier 2 tests, the Medaka Extended One Generation Reproduction Test (MEOGRT) was developed by the USEPA for the EDSP and, in collaboration with the Japanese Ministry of the Environment, for the Organisation for Economic Co-operation and Development’s (OECD) Guidelines for the Testing of Chemicals. The MEOGRT protocol was iteratively modified based upon knowledge gained after successfully completing nine tests with variations in test protocols. The current manuscript describes both the final MEOGRT protocol that has been published by the USEPA and the OECD, the iterations of the protocol that provided valuable insights into nuances of the protocol, and the datasets and summaries from the various tests that informed the decision making process.
