You are here:
Operational Testing of Sporicidal Wipes for Decontamination of Surfaces Contaminated with Bacillus Anthracis Surrogate Spores Addendum
Citation:
Oudejans, L. Operational Testing of Sporicidal Wipes for Decontamination of Surfaces Contaminated with Bacillus Anthracis Surrogate Spores Addendum. U.S. Environmental Protection Agency, Washington, DC, 2017.
Impact/Purpose:
This addendum to the report “Operational Testing of Sporicidal Wipes for Decontamination of Surfaces Contaminated with Bacillus anthracis Surrogate Spores” describes the outcome of additional research that was conducted post publication of the original report (September 2015). It considered the same objectives/goals and uses the same research approaches. Whereas the initial study focused on a medium-size 42 in. x 42 in. surface area, the study presented in this addendum focuses on a smaller 28 in. x 28 in. surface area. The primary objective of the whole project was to evaluate the operational aspects of sporicidal wiping approaches as a decontamination approach using commercially available sporicidal wipes on various materials. The results of the study captured in the main body of this report indicated none of the wipes demonstrated a minimum six log reduction of antimicrobial effectiveness against B. atrophaeus spores as required by the U.S. Federal Insecticide, Fungicide and Rodenticide Act (FIFRA) when used to inactivate spores on a medium size surface area (42 inch (in.) x 42 in.). Spatial distributions of the post decontamination spore concentration on the target coupons, as determined from discrete samples, showed that significant cross contamination occurs when wiping large area, likely due to the drying of the exposed surface area of the sporicidal wipe. Based on these results, an additional series of tests (subject of this addendum), was conducted to evaluate the best performing decontamination wipe (Hype-Wipe® Bleach Towelette) on intermediate size coupons (28 in. x 28 in.) to avoid potential drying out of the exposed surface of the wipe before completing the decontamination process of the entire surface of the coupon. These data will help individuals such as incident commanders and remediation personnel make informed decisions about surface decontamination after a biological contamination incident.
Description:
Report
URLs/Downloads:
Operational Testing of Sporicidal (PDF, NA pp, 4949 KB, about PDF)addendum to Operational Testing of Sporicidal Wipes (PDF, NA pp, 3942 KB, about PDF)