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Optimization of a Sample Processing Protocol for Recovery of Bacillus anthracis Spores from Soil
Silvestri, E., D. Feldhake, D. Griffin, J. Lisle, T. Nichols, S. Shah, A. Pemberton, AND F. Schaefer. Optimization of a Sample Processing Protocol for Recovery of Bacillus anthracis Spores from Soil . JOURNAL OF MICROBIOLOGICAL METHODS. Elsevier Science Ltd, New York, NY, 130:6-13, (2016).
Following a release of Bacillus anthracis spores into the environment, there is a potential for lasting environmental contamination in soils. There is a need for detection protocols for B. anthracis in environmental matrices. However, identification of B. anthracis within a soil is a difficult task. Processing soil samples helps to remove debris, chemical components, and biological impurities that can interfere with microbiological detection. This study aimed to optimize a previously used indirect processing protocol, which included a series of washing and centrifugation steps.
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OPTIMIZATION OF A SAMPLE PROCESSING PROTOCOL FOR RECOVERY OF BACILLUS ANTHRACIS SPORES FROM SOIL Exit