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Monitoring Ecological Impacts of Environmental Surface Waters using Cell-based Metabolomics
Nguyen, H., H. Zhen, D. Ekman, Dan Villeneuve, J. Cavallin, G. Ankley, Tim Collette, P. Bradley, AND Q. Teng. Monitoring Ecological Impacts of Environmental Surface Waters using Cell-based Metabolomics. SETAC North America 37th Annual Meeting, Orlando, FL, November 06 - 10, 2016.
Presented at SETAC North America 37th Annual Meeting
Optimized cell-based metabolomics has been used to study the impacts of contaminants in surface waters on human and fish metabolomes. This method has proven to be resource- and time-effective, as well as sustainable for long term and large scale studies. In the current study, cell-based metabolomics is used to investigate the impacts of contaminants in surface waters on biological pathways in human and ecologically relevant cell lines. Water samples were collected from stream sites nationwide, where significant impacts have been estimated from the most potentially contaminated sources (i.e. waste water treatment plants, concentrated animal feeding operations, mining operations, and plant-based agricultural operations that use intensive chemical applications). Zebrafish liver cells (ZFL) were used to study exposure impacts on in vitro metabolomes. In addition, a small number of water samples were studied using two human cell lines (liver cells, HepG2 and brain cells, LN229). The cellular metabolites were profiled by nuclear magnetic resonance (NMR) spectroscopy and gas chromatography mass spectrometry (GC-MS). Detailed methods and results will be reported.
Record Details:Record Type: DOCUMENT (PRESENTATION/SLIDE)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL EXPOSURE RESEARCH LABORATORY
EXPOSURE METHODS & MEASUREMENT DIVISION