Citation:

Morris, B., H. Buse, N. Adcock, AND E. Rice. A Novel Broth Medium for Enhanced Growth of Francisella Tularensis. Letters in Applied Microbiology. Blackwell Publishing, Malden, MA, 64(6):393-468, (2017).

Impact/Purpose:

Francisella tularensis is a fastidious organism that requires a lengthy incubation time in liquid growth media for detection. The need for achieving detectable viable concentrations of F. tularensis cells in a timely manner is of great importance for development of rapid analytical methods needed during bioterror or natural outbreak responses and for general study of this fastidious bacterium. The objective of this study was to develop a medium formulation using readily available supplements that enhanced early growth of F. tularensis.The addition of selected supplements into the base medium BHI (BVFH) developed in this study enhanced growth of F. tularensis Type A1, A2 and B strains compared to BHI alone. Growth of these organisms in BVFH will allow for improved response time should a natural or intentional contamination event occur.

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Journal article

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