EPA Science Inventory

Processing Protocol for Soil Samples Potentially Contaminated with Bacillus anthracis Spores

Citation:

U.S. EPA. Processing Protocol for Soil Samples Potentially Contaminated with Bacillus anthracis Spores . US Environmental Protection Agency, Cincinnati, OH, 2017.

Description:

Method Operating Procedures

Purpose/Objective:

This protocol describes the processing steps for 45 g and 9 g soil samples potentially contaminated with Bacillus anthracis spores. The protocol is designed to separate and concentrate the spores from bulk soil down to a pellet that can be used for further analysis. Soil extraction solution and mechanical shaking are used to disrupt soil particle aggregates and to aid in the separation of spores from soil particles. Soil samples are washed twice with soil extraction solution to maximize recovery. Differential centrifugation is used to separate spores from the majority of the soil material. The 45 g protocol has been demonstrated by two laboratories using both loamy and sandy soil types. There were no significant differences overall between the two laboratories for either soil type, suggesting that the processing protocol would be robust enough to use at multiple laboratories while achieving comparable recoveries. The 45 g protocol has demonstrated a matrix limit of detection at 14 spores/gram of soil for loamy and sandy soils.

URLs/Downloads:

Processing Protocol for Soil Samples Potentially Contaminated with Bacillus anthracis Spores   (PDF,28 pp, 774 KB,  about PDF)

Record Details:

Record Type: DOCUMENT (PUBLISHED REPORT/METHODOLOGY)
Start Date: 03/09/2017
Completion Date: 03/09/2017
Record Last Revised: 11/14/2017
Record Created: 03/27/2017
Record Released: 03/27/2017
OMB Category: Other
Record ID: 335822

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL HOMELAND SECURITY RESEARCH CENTER

THREAT AND CONSEQUENCE ASSESSMENT DIVISION