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Development and Multi-laboratory Verification of US EPA Method 543 for the Analysis of Drinking Water Contaminants by Online Solid Phase Extraction-LC–MS-MS
Citation:
Shoemaker, J. Development and Multi-laboratory Verification of US EPA Method 543 for the Analysis of Drinking Water Contaminants by Online Solid Phase Extraction-LC–MS-MS. Journal of Chromatographic Science. Preston Publications Incorporated, Niles, IL, 54(9):1532-1539, (2016).
Impact/Purpose:
The journal article describes the development of drinking water Method 543 for analysis of selected CCL 3 chemicals. It is anticipated this method may be used in a future Unregulated Contaminant Monitoring Regulation to gather nationwide occurrence data on the CCL analytes contained in this method.
Description:
A drinking water method for seven pesticides and pesticide degradates is presented that addresses the occurrence monitoring needs of the US Environmental Protection Agency (EPA) for a future Unregulated Contaminant Monitoring Regulation (UCMR). The method employs online solid phase extraction-liquid chromatography–tandem mass spectrometry (SPE-LC–MS-MS). Online SPE-LC–MS-MS has the potential to offer cost-effective, faster, more sensitive and more rugged methods than the traditional offline SPE approach due to complete automation of the SPE process, as well as seamless integration with the LC–MS-MS system. The method uses 2-chloroacetamide, ascorbic acid and Trizma to preserve the drinking water samples for up to 28 days. The mean recoveries in drinking water (from a surface water source) fortified with method analytes are 87.1–112% with relative standard deviations of <14%. Single laboratory lowest concentration minimum reporting levels of 0.27–1.7 ng/L are demonstrated with this methodology. Multi-laboratory data are presented that demonstrate method ruggedness and transferability. The final method meets all of the EPA's UCMR survey requirements for sample collection and storage, precision, accuracy, and sensitivity.
