EPA Science Inventory

Detonation Nanodiamond Toxicity in Human Airway Epithelial Cells Is Modulated by Air Oxidation

Citation:

Silbajoris, R., Bill Linak, O. Shenderova, C. Winterrowd, H. Chang, J. Zweier, A. Kota, L. Dailey, P. Bromberg, AND J. Samet. Detonation Nanodiamond Toxicity in Human Airway Epithelial Cells Is Modulated by Air Oxidation. Diamond and Related Materials. Elsevier B.V., Amsterdam, Netherlands, 58:16-23, (2015).

Description:

Detonational nanodiamonds (DND), a nanomaterial with an increasing range of industrial and biomedical applications, have previously been shown to induce a pro-inflammatory response in cultured human airway epithelial cells (HAEC). We now show that surface modifications induced by air oxidation of DND (AO-DND), including an increase in oxygen content, formation of carboxylic groups associated with the appearance of high negative zeta potential and a decrease in free radical content, are accompanied by a significant loss of bioactivity, as measured by levels of interleukin-8 mRNA in HAEC. These findings are relevant to the identification of chemical determinants and molecular mechanisms of the inhalational toxicity of carbonaceous nanomaterials.

Purpose/Objective:

This study examines the relationship between the surface chemistry of carbonaceous nanomaterials and their biological activity. This is fundamentally relevant to the inhalational toxicology of combustion-derived particulate matter.

Record Details:

Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Completion Date: 09/30/2015
Record Last Revised: 06/03/2016
Record Created: 06/03/2016
Record Released: 06/03/2016
OMB Category: Other
Record ID: 317130

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LAB

ENVIRONMENTAL PUBLIC HEALTH DIVISION

CLINICAL RESEARCH BRANCH