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COMPARISON OF INTRACRANIAL INFUSIONS OF COLCHICINE AND IBOTENIC ACID AS MODELS OF NEURODEGENERATION IN THE BASAL FOREBRAIN
Citation:
Shaughnessy, L., S. Barone, Jr., W. Mundy, D. Herr, AND H. Tilson. COMPARISON OF INTRACRANIAL INFUSIONS OF COLCHICINE AND IBOTENIC ACID AS MODELS OF NEURODEGENERATION IN THE BASAL FOREBRAIN. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-94/318.
Description:
Colchicine and ibotenic acid were compared for their ability to roduce neurodegeneration and cognitive deficit after bilateral infusions into the nucleus basalis magnocellularis of male Long-Evans rats. our weeks post-lesion, there was no difference in locomotor activity following intracranial infusion of either neurotoxicant or vehicle. n a passive avoidance task, both treated groups had significantly shorter step-through latencies compared with controls. ive weeks post-lesion, rats were killed for neurochemistry or histochemistry. hAT activity in both the frontal and parietal cortex was significantly decreased (25-35%) in the colchicine- and ibotenic acid-infused rats when compared to control levels. here was no effect of either neurotoxicant on ChAT activity in the hippocampus or striatum. oth neurotoxicants produced damage in the general area of the ventromedial pallidum, although ibotenic acid infusion consistently produced a larger area of damage as assessed in Nissl-stained sections. nalysis of the number of ChAT-immunoreactive cells in the NBM showed an average 60% cell loss following colchicine infusion and a 76% cell loss after ibotenic acid (51%) and colchicine infusions (28%). olchicine infusion decreased dopamine and DOPAC in the striatum, ibotenic acid had no effect on brain catechol- or indoleamine levels. he results indicate that although similar cholinergic hypofunction and behavioral deficits were achieved, several non-cholinergic differences between the neurotoxicants were detected.