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Multiplex analysis inflammatory cytokines in human blood, breath condensate, and urine matrices
Citation:
Stiegel, M., J. Pleil, J. Sobus, AND M. Madden. Multiplex analysis inflammatory cytokines in human blood, breath condensate, and urine matrices. Presented at PittCon 2014, Chicago, IL, March 02 - 06, 2014.
Impact/Purpose:
The National Exposure Research Laboratory (NERL) Human Exposure and Atmospheric Sciences Division (HEASD) conducts research in support of EPA mission to protect human health and the environment. HEASD research program supports Goal 1 (Clean Air) and Goal 4 (Healthy People) of EPA strategic plan. More specifically, our division conducts research to characterize the movement of pollutants from the source to contact with humans. Our multidisciplinary research program produces Methods, Measurements, and Models to identify relationships between and characterize processes that link source emissions, environmental concentrations, human exposures, and target-tissue dose. The impact of these tools is improved regulatory programs and policies for EPA.
Description:
Scientific evidence suggests that inflammation is associated with human health effects and health endpoints, yet most studies have focused on human populations that are already considered “unhealthy”. As such, it is pertinent to measure inflammatory biomarkers in human biological media with an appropriate sensitivity and specificity for an environmental or low-level exposure. To this end, we used Meso Scale Discovery’s (MSD) MUTIL-SPOT Human TH1/TH2 Cytokine10-Plex Assay Ultra-Sensitive Kit (Gaithersburg, MD) to evaluate the use of this methodology for the investigation of interleukins (1β, 2, 4, 5, 8, 10, 12p70 and 13), IFN- γ, and tumor necrosis factor-α (TNF- α) in human plasma, exhaled breath condensate and urine. We have found that the MSD instrumentation and the TH1/TH2 cytokine 10-plex method have sufficient sensitivity and specificity