EPA Science Inventory

METHOD 544. DETERMINATION OF MICROCYSTINS AND NODULARIN IN DRINKING WATER BY SOLID PHASE EXTRACTION AND LIQUID CHROMATOGRAPHY/TANDEM MASS SPECTROMETRY (LC/MS/MS)

Citation:

Shoemaker, J., Dan Tettenhorst, AND A. Delacruz. METHOD 544. DETERMINATION OF MICROCYSTINS AND NODULARIN IN DRINKING WATER BY SOLID PHASE EXTRACTION AND LIQUID CHROMATOGRAPHY/TANDEM MASS SPECTROMETRY (LC/MS/MS). U.S. Environmental Protection Agency, Washington, DC, 2015.

Description:

Method 544 is an accurate and precise analytical method to determine six microcystins (including MC-LR) and nodularin in drinking water using solid phase extraction and liquid chromatography tandem mass spectrometry (SPE-LC/MS/MS). The advantage of this SPE-LC/MS/MS is its sensitivity and ability to speciate the microcystins. This method development task establishes sample preservation techniques, sample concentration and analytical procedures, aqueous and extract holding time criteria and quality control procedures. Draft Method 544 undergone a multi-laboratory verification to ensure other laboratories can implement the method and achieve the quality control measures specified in the method. It is anticipated that Method 544 may be used in UCMR 4 to collect nationwide occurrence data for selected microcystins in drinking water.

Purpose/Objective:

The purpose of this research project is to develop an accurate and precise analytical method to concentrate and determine selected MCs and nodularin in drinking water.

URLs/Downloads:

METHOD544_FINAL   (PDF,NA pp, 317 KB,  about PDF)

Record Details:

Record Type: DOCUMENT (PUBLISHED REPORT/METHODOLOGY)
Completion Date: 02/24/2015
Record Last Revised: 09/08/2015
Record Created: 02/26/2015
Record Released: 02/26/2015
OMB Category: Other
Record ID: 306953

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL EXPOSURE RESEARCH LAB

MICROBIOLOGICAL AND CHEMICAL EXPOSURE ASSESSMENT DIVISION

CHEMICAL EXPOSURE RESEARCH BRANCH