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ASSESSMENT OF STANDARD REFERENCE COMPOUNDS FOR COMPARATIVE STUDIES USING THE SALMONELLA TYPHIMURIUM MUTAGENICITY ASSAY: I. WITHOUT EXOGENOUS ACTIVATION
Citation:
Claxton, L., V. Houk, L. Monteith, L. Myers, AND T. Hughes. ASSESSMENT OF STANDARD REFERENCE COMPOUNDS FOR COMPARATIVE STUDIES USING THE SALMONELLA TYPHIMURIUM MUTAGENICITY ASSAY: I. WITHOUT EXOGENOUS ACTIVATION. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-91/263.
Description:
Finney (1978) described a bioassay as an experiment for estimating the nature, constitution, or potency of a material by means of the eaction that follows its application to living matter. n this paper, two independent laboratories tested 10 known Salmonella mutagens in order to determine if the selected chemicals potentially could be used as standard reference materials. f variance within a bioassay is sufficiently low and the rankings are of acceptable consistency, the chemicals could be evaluated for use as standard control compounds, as audit materials, and as standard reference materials for comparative bioassay efforts. he results demonstrated that the chosen chemicals (with the possible exception of dimethylcarbamylchloride) provide such consistent results in the Salmonella mutagenicity bioassay that they can be used for bioassay controls, as special audit chemicals, and potentially as reference standards in comparative bioassay efforts. owever, reference standards, whether used as audit materials or in comparative bioassays, should be used concurrently with the test substances of interest; used without bias; be used in a standardized, highly controlled bioassay; and be tested across an appropriate dose range. he study also shows that when these compounds are used as reference standards much care must be given to the number and spacing of doses if highly reproducible slope values are to be generated. e recommend that a pilot test be used to establish a dose range for definitive tests and that doses for definitive tests be placed within the first half of the linear dose response curve. t least two replicate tests should be completed with the defined dose range and results should be analyzed in a non-biased statistical manner.