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Developing exposure indices of graphene-based nanoparticles by coupling lipid-membrane interactions and in vitro cellular response
Henderson, Matt, I. Chowdhury, X. Chang, Q. Teng, AND D. Bouchard. Developing exposure indices of graphene-based nanoparticles by coupling lipid-membrane interactions and in vitro cellular response. Presented at 248th ACS National Meeting, San Francisco, CA, August 11 - 15, 2014.
Presented at the 248th ACS National Meeting.
Graphene-based nanoparticles (NPs) are used extensively in industrial, consumer, and mechanical applications based on their unique structural properties. Due to increasing use of these NPs, environmental exposure to graphene oxide (GO) is probable. GO has been shown to compromise cell integrity via interactions with lipid membranes and subsequent induction of oxidative stress in vitro. An understanding of GO-membrane interactions and resulting potential of GO to perturb biological systems is crucial for estimating risk. To this end, a biomarker-based approach was employed to correlate fluxes in cellular metabolites with altered mechanical properties of cell membranes following GO exposure. Furthermore, the interaction of GO with artificial lipid membranes and vesicles was investigated to aid in developing methods to predict the exposure potential of these NPs. Ultimately, these data will aid in identifying potential biomarkers of GO exposure and facilitate the development of exposure indices of GO in human and ecologically relevant species.
Record Details:Record Type: DOCUMENT (PRESENTATION/SLIDE)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL EXPOSURE RESEARCH LABORATORY
ECOSYSTEMS RESEARCH DIVISION