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Rapid-Viability PCR Method for Detection of Live, Virulent Bacillus anthracis in Environmental Samples
Le´tant, S. E., G. A. Murphy, T. M. Alfaro, J. R. Avila, S. R. Kane, E. Raber, T. M. Bunt, AND S. Shah. Rapid-Viability PCR Method for Detection of Live, Virulent Bacillus anthracis in Environmental Samples. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. American Society for Microbiology, Washington, DC, 77(18):6570-6578, (2011).
The method, referred to as rapid-viability (RV)-PCR, uses the change in cycle threshold after incubation to detect the presence of live organisms. In this article, we report a novel RV-PCR method for detection of live, virulent Bacillus anthracis.