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Effect of pH on the Electrophoretic Mobility of Spores of Bacillus anthracis and Its Surrogates in Aqueous Solutions
White, C. P., J. Popovici, D. A. Lytle, N. J. Adcock, AND E. W. Rice. Effect of pH on the Electrophoretic Mobility of Spores of Bacillus anthracis and Its Surrogates in Aqueous Solutions . APPLIED AND ENVIRONMENTAL MICROBIOLOGY. American Society for Microbiology, Washington, DC, 78(23):8470-8473, (2012).
Electrophoretic mobility (EPM) of endospores of Bacillus anthracis and surrogates were measured in aqueous solution across a broad pH range and several ionic strengths. EPM values trended around phylogenetic clustering based on the 16S rRNA gene. Measurements reported here provide new insight for Bacillus anthracis surrogate selection, attachment/detachment and transport studies.
Measurements of endospores of virulent strains of B. anthracis are needed to fully evaluate the data presented here in the context of surrogate selection for virulent strains. Though a ‘panacea surrogate’ for all types of experimentation on B. anthracis may not exist, a well characterized group of surrogates will help researchers choose the appropriate surrogate species for their particular study. The data presented here should help researchers interpret results obtained in studies requiring the use of surrogates as well as aiding in the selection of ‘worst-case’ surrogates.
Record Details:Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL RISK MANAGEMENT RESEARCH LABORATORY
WATER SUPPLY AND WATER RESOURCES DIVISION
TREATMENT TECHNOLOGY EVALUATION BRANCH