You are here:
Effects of developmental exposure to a Commercial PBDE mixture (DE-71) on protein networks in the rat Cerebellum and Hippocampus
Citation:
Winnik, W., J. Royland, C. Osorio, O. Alzate, AND P. Kodavanti. Effects of developmental exposure to a Commercial PBDE mixture (DE-71) on protein networks in the rat Cerebellum and Hippocampus. Presented at American Society for Mass Spectrometry Annual Conference, Minneapolis, MN, June 09 - 13, 2013.
Impact/Purpose:
Improved protein identification and used it in the larger NHEERL project pursed by Dr. P. Kodavanti. The analysis was done in the NHEERL Research Core Unit.
Description:
Title (20 words): Effects of developmental exposure to a Commercial PBDE mixture (DE-71) on protein networks in the rat Cerebellum and Hippocampus. Introduction (120 words): Polybrominated diphenyl ethers (PBDE5) possess neurotoxic effects similar to those of PCBs. The cellular and molecular mechanisms for these neurotoxic effects are not fully understood; however, several studies have shown that PBDEs affect thyroid hormones, cause oxidative stress, and disrupt Ca2-mediated signal transduction. A variety of these signal transduction pathways lead to changes in gene regulation and down-stream protein expression, which can affect the development and function of the nervous system. In this study, we examined protein expression profiles in the cerebellum (Cb) and hippocampus (Hip) of rat pups exposed to the commercial PBDE mixture, DE-71. An improved MALDI-MS and MS/MS technique of protein identification from 2D gels was applied to the analytically-challenging brain proteins resolved on 2D-gels.