Science Inventory

Multi-Laboratory Validation of Estrone (E1) ELISA Methods

Citation:

Rubio, F., D. Russell, E. Kleiner, A. Tang, A. Lincoff, L. Webb, B. Kim, AND R. Schneider. Multi-Laboratory Validation of Estrone (E1) ELISA Methods. Presented at PittConn, Philadelphia, PA, March 17 - 21, 2013.

Impact/Purpose:

To inform the scientific community.

Description:

This project is a round-robin evaluation of commercially available Enzyme-Linked Immunosorbent Assay (ELISA) technology to quantitatively or qualitatively measure the hormone estrone (E1) in combined animal feeding operation (CAFO) receiving streams. ELISA is meant to be a simple, user-friendly technology that uses competition between enzyme labeled conjugates and the target analyte in the sample to bind with a limited number of antibody binding sites on a plate. The target compound concentration is determined by a color change measured on a standard plate reader.

URLs/Downloads:

http://pittcon.org/wp-content/uploads/2013/03/Pittcon-2013-Final-Program.pdf   Exit

Record Details:

Record Type: DOCUMENT (PRESENTATION/SLIDE)
Product Published Date: 03/17/2013
Record Last Revised: 05/22/2013
OMB Category: Other
Record ID: 254890

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL RISK MANAGEMENT RESEARCH LABORATORY

WATER SUPPLY AND WATER RESOURCES DIVISION

WATER QUALITY MANAGEMENT BRANCH