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Behind the scene with the fathead team: Part II. In-lab flow through exposures and “omics” to complement effects based monitoring in the Duluth-Superior Harbor.
Citation:
Stevens, K., J. Cavallin, M. Severson, J. Berninger, E. Durhan, E. Eid, K. Jensen, M. Kahl, C. LaLone, L. Makynen, Dan Villeneuve, AND G. Ankley. Behind the scene with the fathead team: Part II. In-lab flow through exposures and “omics” to complement effects based monitoring in the Duluth-Superior Harbor. Presented at Midwest SETAC, March 19 - 20, 2013.
Impact/Purpose:
not applicable
Description:
As part of a research team focused on aquatic toxicity testing using fathead minnows as a model species, this presentation is the second of a three-part series, giving an overview of the types of field and laboratory studies as well as sample processing our team conducts at the U.S. EPA, Mid-Continent Ecology Divison. “Part II: In-lab flow through exposures and “omics” to complement effects based monitoring in the Duluth-Superior Harbor.” describes technical aspects of lab based exposures and sample collection. As an extension of a presumed concentration gradient established for an in situ exposure in the Duluth-Superior harbor, concurrent in-lab flow-through exposures to varying dilutions of final treated effluent collected from the Western Lake Superior Sanitary District were conducted. Additionally, fish were exposed to a single concentration of bisphenol A (10 µg/L), a chemical of emerging concern, previously detected in the effluent and receiving water. Tissue samples including mucus, liver, gonads and urine were collected and analyzed using open-ended or “unsupervised” tools including metabolomics, and transcriptomics. Results from these approaches compliment more specific “supervised” endpoints associated with known adverse outcome pathways, using biochemical and molecular markers of endocrine disruption (VTG transcription/translation, testosterone/estrogen production, etc), detailed in part III of this series.