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Behind the scene with the fathead team: Part III. Molecular, biochemical, and in vitro analyses
Citation:
Severson, M., J. Cavallin, K. Stevens, J. Berninger, E. Durhan, E. Eid, K. Jensen, M. Kahl, C. LaLone, L. Makynen, Dan Villeneuve, AND G. Ankley. Behind the scene with the fathead team: Part III. Molecular, biochemical, and in vitro analyses. Presented at Midwest SETAC, March 19 - 20, 2013.
Impact/Purpose:
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Description:
As part of a research team focused on aquatic toxicity testing using fathead minnows as a model species, this presentation is the third in the three-part series, giving an overview of the types of field and laboratory studies as well as sample processing our team conducts at the U.S. EPA, Mid-Continent Ecology Divison. “Part III: Molecular, biochemical, and in vitro analyses” details the procedures used to analyze surface water samples as well as tissues and bio-fluids from fathead minnows (e.g. gonad, plasma, liver, and carcass.) The analyses employed include: ex-vivo steriodogenesis assay, radioimmunoassay (RIA), quantitative polymerase chain reaction (QPCR), histology, enzyme linked immunosorbent assay (ELISA), analytical residual analysis, and cell bioassays. Results from recent experiments will be presented to highlight the complementary utility of the various assays and endpoints. The data collected from this battery of assays provides a means to evaluate exposures to contaminants of emerging concern (CECs) in the field, quantify effects, and define and describe endocrine adverse outcome pathways.