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Temporal Profile of Gene Expression Alterations in Primary Human Bronchial Epithelial Cells Following In Vivo Exposure to Ozone
Citation:
Duncan, K., J. Crooks, D. Miller, L. Burgoon, Mike Schmitt, S. Edwards, D. Diaz-Sanchez, AND R. Devlin. Temporal Profile of Gene Expression Alterations in Primary Human Bronchial Epithelial Cells Following In Vivo Exposure to Ozone. Presented at American Thoracic Society (ATS) Meeting, May 17 - 22, 2013.
Impact/Purpose:
This abstract will be presented at the American Thoracic Society (ATS) Meeting, Phildelphia, PA, May 17-22, 2013
Description:
RATIONALE: Ozone (Os) isa ubiquitous air pollutant that has been shown to have a detrimental effect on human health. Controlled exposure studies in humans have demonstrated that acute exposure to 03 results in reversible reduction in lung function immediately post-exposure, increased lung injury and inflammation and reduced host-defense capabilities. Although the physiological effects to 03 are well established, the effect of O on bronchial epithelial cells (BEC) and the resultant contribution of the airway epithelium to these acute responses are not clearly defined. The purpose of this study is to evaluate the temporal profile of gene expression alterations in BEC following in vivo exposure to 03.
METHODS: Healthy adult human volunteers were randomly exposed to filtered air (FA) and 0.3 ppm O for 2 h with intermittent exercise with a minimum of four weeks between exposures. A bronchoscopy procedure was conducted either 24 h (n=23) or 1 h (n=9) post-exposure to collect BEC through brush biopsies of the mainstem bronchus. Whole genome gene expression data were obtained using the Alumina Human HT-12v4 Beadchips and were analyzed using an eBayes-moderated t-statistic with subsequent multiple test correction for the identification of differentially-expressed (DE) genes between the O- and FA-exposeci BEC at each time point post-exposure. RESULTS: At 1 h post-exposure, a total of 203 probes corresponding to 163 named genes were significantly DE (I FCI>1.3, q