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A field-based approach for assessing the impact of paper pulp mill effluent on the metbolite profile of fathead minnows (Pimephales promelas)
Citation:
Davis, J., Tim Collette, D. Ekman, G. Ankley, J. Cavallin, K. Jensen, M. Kahl, AND D. Villeneuve. A field-based approach for assessing the impact of paper pulp mill effluent on the metbolite profile of fathead minnows (Pimephales promelas). Presented at SETAC North America 33rd Annual Meeting, Long Beach, CA, November 11 - 15, 2012.
Impact/Purpose:
Poster presented at SETAC North America 33rd Annual Meeting
Description:
Although evidence indicates that exposure to effluent from paper pulp mills (PME) can alter the body condition, secondary sexual characteristics, and reproductive success of aquatic organisms, there is currently little understanding of the biochemical mechanisms for these effects (e.g., changes in endogenous metabolites). Here, we present results from a field-based study that assessed PME exposure on the polar metabolite profile of fathead minnows (Pimephales promelas) during a temporary shutdown of a kraft pulp mill. Male and female fathead minnows were deployed in cages for 48-hrs during three separate time periods (pre-pulp mill shutdown, during pulp mill shutdown, and after the pulp mill was restarted) at two locations in the Duluth-Superior Harbor of Lake Superior, USA (10- and 200-m from a waste-water treatment discharge that is typically around 45% PME). Although evidence indicates that exposure to effluent from paper pulp mills (PME) can alter the body condition, secondary sexual characteristics, and reproductive success of aquatic organisms, there is currently little understanding of the biochemical mechanisms for these effects (e.g., changes in endogenous metabolites). Here, we present results from a field-based study that assessed PME exposure on the polar metabolite profile of fathead minnows (Pimephales promelas) during a temporary shutdown of a kraft pulp mill. Male and female fathead minnows were deployed in cages for 48-hrs during three separate time periods (pre-pulp mill shutdown, during pulp mill shutdown, and after the pulp mill was restarted) at two locations in the Duluth-Superior Harbor of Lake Superior, USA (10- and 200-m from a waste-water treatment discharge that is typically around 45% PME). Additional fish were exposed to pristine Lake Superior water for 48-hrs in flow-through aquaria under lab conditions. We then used 1H-NMR spectroscopy to measure polar metabolites in livers and subsequently applied principal component analysis (PCA) to
