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Using in Vitro High Throughput Screening Assays to Identify Potential Endocrine-Disrupting Chemicals
Citation:
ROTROFF, D. M., D. J. DIX, K. A. HOUCK, T. B. KNUDSEN, M. T. MARTIN, K. M. MCLAURIN, D. M. REIF, K. M. CROFTON, A. V. SINGH, M. XIA, R. HUANG, AND R. S. JUDSON. Using in Vitro High Throughput Screening Assays to Identify Potential Endocrine-Disrupting Chemicals. ENVIRONMENTAL HEALTH PERSPECTIVES. National Institute of Environmental Health Sciences (NIEHS), Research Triangle Park, NC, Open Access(Epub):1-39, (2012).
Impact/Purpose:
Here we investigate using EPA ToxCast HTS assays for estrogen, androgen, steroidogenic, and thyroid disrupting mechanisms to classify compounds, and compare ToxCast results to in vitro and in vivo data from EDSP T1S assays. Overall, results suggest that current ToxCast assays can accurately identify chemicals with potential to interact with the estrogenic and androgenic pathways, and could help prioritize chemicals for EDSP T1S assays.
Description:
Over the past 20 years, an increased focus on detecting environmental chemicals posing a risk of adverse effects due to endocrine disruption has driven the creation of the U.S. EPA Endocrine Disruptor Screening Program (EDSP). Thousands of chemicals are subject to the EDSP, which could require millions of dollars and decades to process using current test batteries. A need for increased throughput and efficiency motivated the development of methods using in vitro high-throughput screening (HTS) assays to prioritize chemicals for EDSP Tier 1 screening (TIS).
