EPA Science Inventory

Influences of sample interference and interference controls on quantification of enterococci fecal indicator bacteria in surface water samples by the qPCR method

Citation:

HAUGLAND, R. A., S. SIEFRING, J. Lavender, AND M. VARMA. Influences of sample interference and interference controls on quantification of enterococci fecal indicator bacteria in surface water samples by the qPCR method. WATER RESEARCH. Elsevier Science Ltd, New York, NY, 46(18):5989-6001, (2012).

Description:

A quantitative polymerase chain reaction (qPCR) method for the detection of entercocci fecal indicator bacteria has been shown to be generally applicable for the analysis of temperate fresh (Great Lakes) and marine coastal waters and for providing risk-based determinations of water quality at recreational beaches.

Purpose/Objective:

1) Develop and evaluate qPCR assays and test methods for the detection and quantification of genetic markers from indicator bacteria that are associated with human fecal waste and from two new groups of general fecal indicator bacteria (E. coli and Clostridia) that historically have been widely used or are favored in specific regions 2) Determine the occurrence and densities of genetic markers detected by new qPCR assays developed under objective 1 and compare with occurrence and densities of genetic markers detected by previously developed qPCR assays for enterococci and total Bacterioidalesin waste waters and fecal material from different animal sources. 3) Determine stability of fecal indicator bacteria target DNA sequences in freezer archived filter retentates of ambient surface water samples 4) Determine the densities of human and general fecal indicator markers in a wide range of surface and recreational waters including archived samples from previous NEEAR studies.

URLs/Downloads:

j.watres.2012.08.017   Exit

Record Details:

Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Start Date: 12/30/2012
Completion Date: 12/30/2012
Record Last Revised: 05/03/2013
Record Created: 04/02/2012
Record Released: 04/02/2012
OMB Category: Other
Record ID: 242289

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY

OFFICE OF RESEARCH AND DEVELOPMENT

NATIONAL EXPOSURE RESEARCH LAB

MICROBIOLOGICAL AND CHEMICAL EXPOSURE ASSESSMENT DIVISION

MICROBIAL EXPOSURE RESEARCH BRANCH