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Use of Acid Treatment and a Selective Medium to Enhance the Recovery of Francisella tularensis from Water
Humrighouse, B. W., N. J. ADCOCK, AND E. W. Rice. Use of Acid Treatment and a Selective Medium to Enhance the Recovery of Francisella tularensis from Water. Sept 2011 APPLIED AND ENVIRONMENTAL MICROBIOLOGY. American Society for Microbiology, Washington, DC, 77(18):6729-6732, (2011).
Francisella tularensis has been associated with naturally occurring waterborne outbreaks and is also of interest as a potential biological wespon. Recovery of this pathogen from water using cultural methods is challenging due to the organism's growth requirements and interference by indigenous bacteria. An acid treatment procedure prior to culture on a selective agar was evaluated for recovery of F. tularensis from seeded water samples. The acid treatment procedure coupled with plating on cystine heart agar with rabbit blood and antiibiotics (CHARBab) allowed for the isolation of F. tularensis seeded to five natural water samples.
URLs/Downloads:USE OF ACID TREATMENT AND A SELECTIVE MEDIUM TO ENHANCE THE RECOVERY OF FRANCSELLA TULARENSIS FROM WATER Exit
Record Details:Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL HOMELAND SECURITY RESEARCH CENTER
WATER INFRASTRUCTURE PROTECTION DIVISION