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Proteomic analysis of zebrafish brain tissue following exposure to the pesticide prochloraz
Citation:
BIALES, A. D., D. C. BENCIC, DAN VILLENEUVE, G. T. ANKLEY, AND D. L. LATTIER. Proteomic analysis of zebrafish brain tissue following exposure to the pesticide prochloraz. AQUATIC TOXICOLOGY. Elsevier Science Ltd, New York, NY, 105(3-4):618-628, (2011).
Impact/Purpose:
Proposed research will provide environmental science and risk assessment communities with advanced molecular biological indicator methods, complete with validation studies and guidance for their application.
Description:
The hypothalamus-pituitary-gonadal (HPG) axis plays a central role in the maintenance of homeostasis. Disruptions of this axis can have important implications for development and other critical biological processes. A number of compounds found in aquatic environments are known to affect the HPG axis. In the present study, we used 2-dimensional electrophoresis to interrogate the proteome of female and male zebrafish brain following a 96 h exposure to the fungicide prochloraz. Prochloraz is known to impact a number of HPG foci, including production of steroidogenic hormones, and has been used as a model chemical for disruption of HPG function. Twenty-eight proteins were identified as differentially expressed in females and 22 in males. Proteins were identified using LC-MS/MS and identities were examined relative to brain function in the context of changing steroid hormone levels. Proteins with known roles in metabolism, learning, neuroprotection, and calcium regulation were found to be differentially regulated. We also used differentially expressed proteins to establish a classifier consisting of three proteins that was able to discriminate between PCZ-exposed and control females. Brain functions that were impacted and specific protein changes that were observed may be generalizable to other chemicals that work through the same mode(s) of action.