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Serum biomarkers in young adult and aged Brown Norway (BN) rats following episodic (weekly) ozone exposure
GORDON, C. J., J. Schmid, J. R. Ledbetter, W. W, M. SCHLADWEILER, K. Jarema, U. P. KODAVANTI, AND R. C. MACPHAIL. Serum biomarkers in young adult and aged Brown Norway (BN) rats following episodic (weekly) ozone exposure. Presented at Experimental Biology Annual Meeting, Washington, DC, April 09 - 13, 2011.
Ozone (03) is an air pollutant that is associated with cardiovascular and respiratory diseases. Older adults are considered to be particularly susceptible to oxidant air pollutants such as 03. Serum biomarkers are being sought that would lead to better predictions of susceptibility in the aged to air pollutants. We exposed young adult (4 month) and aged (20 month) male BN rats (N=7) to air (control) or 0.8 ppm 03 for 6 hr/d, 1 d/wk for 17 wks. Respiratory parameters assessed by unrestrained plethysmography the day after exposure revealed that 03 led to airway-flow limitation. Rats were terminated 24 hr after the last exposure. Bronchoalveolar fluid macrophages were reduced in aged rats and neutrophils increased in young rats exposed to 03. A panel of 58 protein analytes was quantified in serum, including cytokines, chemokines, and tissue factors (Rules Based Medicine). Age affected 20 analytes, including C-reactive protein, interferon, factor VII, and macrophage inflammatory protein-2 (MIP-2). 03 affected 6 analytes, including fibroblast growth factor (basic), MIP-2, leukemia inhibitor factor, and haptoglobin. Age by 03 interactions were obtained for MIP-2 and myoglobin. The data illustrate that serum analytes may be beneficial as biomarkers to assess aging and susceptibility to criteria air pollutants. This is an abstract of a proposed presentation and does not reflect USEPA policy.
The data illustrate that serum analytes may be beneficial as biomarkers to assess aging and susceptibility to criteria air pollutants.
Record Details:Record Type: DOCUMENT (PRESENTATION/ABSTRACT)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LAB
TOXICOLOGY ASSESSMENT DIVISION