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A Method for the Determination of Genetic Sex in the Fathead Minnow, Pimephales promelas, to Support Testing of Endocrine-active Chemicals
Citation:
OLMSTEAD, A. W., DAN VILLENEUVE, G. T. ANKLEY, J. CAVALLIN, A. LINDBERG-LIVINGSTON, L. C. WEHMAS, AND S. J. DEGITZ. A Method for the Determination of Genetic Sex in the Fathead Minnow, Pimephales promelas, to Support Testing of Endocrine-active Chemicals. ENVIRONMENTAL SCIENCE AND TECHNOLOGY. John Wiley & Sons, Ltd., Indianapolis, IN, 45(7):3090-3095, (2011).
Impact/Purpose:
This research describes the development of a PCR method that can determine the genetic sex in this species.
Description:
Fathead minnows are used as a model fish species for the characterization of the endocrine-disrupting potential of environmental contaminants. This research describes the development of a PCR method that can determine the genetic sex in this species. This method, when incorporated into endocrine disruptor testing, enables the detection of sex reversals in exposed, developing fish. It also allows for investigation into the basic biology or early gonad differentiation in this species at stages in which the gonads are morphologically well defined in the sexes.
URLs/Downloads:
A Method for the Determination of Genetic Sex in the Fathead Minnow, Pimephales promelas, to Support Testing of Endocrine-active Chemicals