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Fireflies in the Coalmine: Luciferase Technologies in Next-Generation Toxicity Testing
Citation:
SIMMONS, S. Fireflies in the Coalmine: Luciferase Technologies in Next-Generation Toxicity Testing. Combinatorial Chemistry & High Throughput Screening. Bentham Science Publishers, Ltd., Oak Park, IL, 14(8):688-702, (2011).
Impact/Purpose:
This article highlights the various uses of luciferase-based technologies in toxicology testing with a focus on high-throughput screening applications and opportunities for assay development.
Description:
Whole-animal studies have been the mainstay of toxicity testing for decades. These approaches are too expensive and laborious to effectively characterize all of the chemicals currently in commercial use. In addition, there are social and ethical pressures to reduce, refine and replace animal testing in toxicology. The National Research Council (NRC) has outlined a new strategy to transition from animal-based tests to highthroughput, cell-based assays and computational modeling approaches to characterize chemical toxicants. Critical to this vision, assays that measure toxicity pathways associated with adverse health effects must be developed. Bioluminescent assays are particularly well suited to the demands of next-generation toxicity testing because they measure a wide range of biological activities in a quantitative and high-throughput manner. Here, I review the limitations of traditional, animal-based toxicity testing and the current and developing uses of bioluminescent technologies in next-generation testing based on three general assay formats: luciferase-limited assays, ATP-limited assays and luciferin-limited assays. This document has been reviewed by the National Health and Environmental Effects Research Laboratory of US EPA and approved for publication. Approval does not signify that the contents reflect the views of the Agency, nor does mention of trade names or commercial products constitute the endorsement of recommendation for use.
