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Use of exhaled breath condensate endpoints for examination of Body Mass Index as a susceptibility factor to diesel exhaust.
Citation:
MADDEN, M. C., J. D. PLEIL, AND K. Sawyer. Use of exhaled breath condensate endpoints for examination of Body Mass Index as a susceptibility factor to diesel exhaust. Presented at Exhaled Breath Analysis from Sensors to Devices and Applications Conference, Barga, ITALY, October 24 - 29, 2010.
Impact/Purpose:
We examined lung responses using the exhaled breath condensate (EBC) of subjects exposed to diesel exhaust (DE).
Description:
High and low Body Mass Index (BMI) is a risk factor for effects (e.g., premature mortality) induced by exposure to common air pollutants such as ozone and particulate matter. Diesel exhaust contributes to particulate matter levels. We examined lung responses using the exhaled breath condensate (EBC) of subjects exposed to diesel exhaust (DE). EBC was collected from ten healthy adolescent subjects either pre-, immediately post-, and 20 hr post controlled exposure to clean filtered air or DE (100 ug particle mass/nr'). The subject BMI range was 21 to 28. The pro-inflammatory cytokine ILl-beta was the only cytokine (out of 10) detectable in the majority of samples, and was detectable primarily at 20 hr post exposure. Comparison of raw values and total protein concentration-normalized values did not suggest a change in the EBC IL I-beta levels in response to DE, nor were the levels correlated with subject BMI. Examination of EBC calcium levels showed changes immediately post DE compared to air that were not correlated with subject BMI. These data suggest that BMI did not affect lung calcium or ILl-beta responses measured in EBC at least for the range examined. Calcium levels were highly correlated with total protein concentrations for all EBC samples (n=57). A wider, extreme range of BMI may be needed to observe changes in EBC parameters induced by DE exposure. [This is an abstract of a proposed presentation and may not reflect official US EPA policy.]