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Oxidative stress studies of six Ti02 and two Ce02 nanomaterials: Immune-spin trapping results with DNA
KITCHIN, K. T., R. Prasad, AND K. WALLACE. Oxidative stress studies of six Ti02 and two Ce02 nanomaterials: Immune-spin trapping results with DNA. Journal of Nanotoxicology. Taylor and Francis, Philadelphia, PA, 5(4):546-56, (2011).
Six Ti02 and two Ce02 nanomaterials with dry sizes ranging from 6 to 410 nm were tested for their ability to cause DNA centered free radicals in vitro in the concentration range of 10 to 3,000 ug/ml. All eight of the nanomaterials significantly increased the adduction of the spin trap agent 5,5-dimethyl-1pyroline N-oxide (DMPO) to DNA as measured by the experimental technique of imrnuno-spln trapping. The eight nanomaterials differed considerably in their potency, slope, and active concentration. The largest increase in DNA nitrone adducts was caused by a Ti02 nanomaterial (25 nm, anatase) from Alfa Aesa. Some nanomaterials that increased the amount of DNA nitrone adducts at the lowest exposure concentrations (100 ug/ml) were Degussa Ti02 (31 nm), Alfa Aesa Ti02(25 nm, anatase) and Nanoamor Ce02 (8 nm, cerianite). At exposure concentrations of 10 or 30 ug/ml, no nanomaterials showed significant in vitro formation of DNA nitrone adducts.
We know little about nanomaterials and their health effects. This study was done to see of nanomaterials could adversely effect DNA and if different nanomaterials differed in their biological potency to do this.
Record Details:Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LAB
INTEGRATED SYSTEMS TOXICOLOGY DIVISION
GENETIC AND CELLULAR TOXICOLOGY BRANCH