Citation:

REICHMAN, J. R., B. SMITH, J. P. Londo, M. A. BOLLMAN, C. A. Auer, AND L. S. WATRUD. Diallelic nuclear microsatellites for diversity and population analyses of the allotraploid creeping bentgrass (Agrostis stolonifera). Crop Science. Crop Science Society of America, Madison, WI, 51:747-758, (2011).

Impact/Purpose:

Nine diallelic nuclear microsatellite loci were characterized in creeping bentgrass, Agrostis stolonifera L. (2n=4x=28, A2A2A3A3).

Description:

Nine diallelic nuclear microsatellite loci were characterized in creeping bentgrass, Agrostis stolonifera L. (2n=4x=28, A2A2A3A3). Markers were developed for analyses of diversity and population biology of A. stolonifera. They may also help with identification of germplasm resources useful for breeding of bentgrass cultivars. Polymorphic loci were isolated from genomic libraries from a wild-collected creeping bentgrass population, a commercial creeping bentgrass cultivar and from an accession of A. transcaspica Litv. (2n=2x=14, putative A3A3). Markers were characterized in 87 A. stolonifera individuals sampled from six distinct sources. Bayesian population structure analyses and groupings based on Rogers’ modified genetic distance resolved wild from commercial genotypes. Each method assigned individuals to clusters that corresponded to their sources. Also, markers correctly identified known intraspecific creeping bentgrass F1 hybrids. Cross-species analyses were conducted on sexually compatible relatives A. gigantea R. (2n=6x=42, A1A1A2A2A3A3), A. capillaris L. (2n=4x=28, A1A1A2A2) and A. canina L. (2n=2x=14, A1A1 or possibly A2A2), plus A. transcaspica. Seven markers were diallelic in A. gigantea. Six of these markers were also diallelic in A. transcaspica, but not other species, suggesting that these loci are A3 genome specific. Results also support the hypothesis that A. transcaspica is the source of the A3 subgenome found in A. stolonifera and A. gigantea.

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